Sequencing Sample Files

This is a group of sample files from one pGEM (control plasmid) sequencing run. The purpose of posting them are:
  1. Provide you with a chance to evaluate the quality of our sequencing runs.
  2. Provide you with a chance to practice the result viewing with packaged files. Mac users can also practice the file conversion of the PC based electropherogram to the Mac format.
  1. Non-packaged pGEM sequencing result files (please click on them to down
    load and save them to your hard disk for further viewing):

pGEM_(ABI)_A01.ab1    (electropherogram, view with Chromas on PC, 
                                                     or with Editview on Mac after file conversion)
pGEM_(ABI)_A01.seq     (text sequence file, view with Notepad or MS Word)

  1. Packaged pGEM sequencing result files (for practicing unpacking):

pGEM.zip

The two files in "I" [pGEM_(ABI)_A01.ab1 and pGEM_(ABI)_A01.seq] are packaged into one file: pGEM.zip. Please follow the following instructions to expand and view your results:

  1. If you prefer to view them on a PC,

  1. Save the file pGEM.zip to your hard disk.
  2. Expand it with StuffIt Expander. Free download:
    http://www.aladdinsys.com/expander/expander_win_login.html
  1. Download the Windows version StuffIt Expander
  2. Install the StuffIt Expander
  3. Start the StuffIt Expander program you just installed
  4. Choose File, Open; then choose to open the pGEM.zip file that you just saved on your hard disk. A folder named pGEM containing the unpacked files will be generated at the same place where the pGEM.zip file is.
  1. View the electroperogram with Chromas. Free download:
    http://www.technelysium.com.au/chromas14x.html 
  1. If you prefer to view your result on a Mac,

  1. Save the file pGEM.zip to your hard disk
    you probably already have the necessary software on your Mac, but if not,
  2. Expand the StuffIt file using the following free download:
    http://www.aladdinsys.com/expander/expander_mac_login.html
  1. Download the Mac version StuffIt Expander
  2. Install the StuffIt Expander
  3. Start the StuffIt Expander program you just installed
  4. Choose File, Open; then choose to open the pGEM.zip file that you just saved on your hard disk.
    A folder named pGEM containing the unpacked files will be generated at the same place where the pGEM.zip file is.
  1. Convert the pGEM_(ABI)_A01.ab1 file to the Mac version (Click here for file conversion instructions)
  2. view the electropherogram with Editview. Free download:
    http://www.appliedbiosystems.com/support/software

        

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Last modified: July 9, 2009